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Rapid Bioassay for Pesticide Residues (RBPR)Reagent Kit

We understand that food safety is non-negotiable. This kit is specifically designed for rapid screening of agricultural products. Through a highly sensitive bio-enzyme reaction, it can effectively detect the presence of high-risk organophosphate and carbamate pesticide residues in fruits and vegetables in a short time. It establishes an efficient food safety protection network without the need for expensive, large-scale equipment.

Rapid Bioassay for Pesticide Residues (RBPR) Reagent Kit

The first line of defense in food safety:

rapid, sensitive, and accurate screening for highly toxic nerve agents.

1. Product Overview

We understand that food safety is non-negotiable. This kit employs internationally recognized biochemical methods and is specifically designed for the rapid screening of agricultural products. Utilizing highly sensitive bio-enzyme reactions, it can effectively detect the presence of high-risk organophosphate and carbamate pesticide residues in fruits and vegetables within a short time. It establishes a highly efficient food safety protection network without requiring expensive, large-scale equipment.

2. Key Features

  • High-sensitivity detection: Purified housefly acetylcholinesterase (AChE) is used, which is highly sensitive to pesticides and capable of detecting even trace amounts of residues.
  • Targeting highly toxic pesticides: Screening is specifically conducted for organophosphate and carbamate pesticides, which are among the most toxic and harmful to the human nervous system.
  • Easy and quick to operate: Standardized operating procedures enable non-professionals to begin with minimal training, significantly lowering the inspection threshold and reducing time costs.
  • Instrument Interpretation: The system is equipped with dedicated pesticide residue detectors (iRBPR-600, UV-1000S, Multiskan FC) that convert color reactions into precise digital data (inhibition rate %), effectively recording and tracking every test result.

3. How it Works

This reagent exploits the properties of acetylcholinesterase (AChE), a neurotransmitter enzyme present in living organisms.

When fruit and vegetable extracts contain organophosphates or carbamates, these pesticides inhibit acetylcholinesterase (AChE) activity, preventing the enzyme from reacting with the colorimetric reagent.

  • No pesticide residue detected (qualified): Normal enzyme activity results in a significant color change of the reagent.
  • Pesticide residues present (unqualified): Enzyme activity is inhibited, resulting in no change in reagent color or a weak reaction.

4. Applications

This product is ideal for organizations conducting self-screening

  • Agricultural product distribution centers and fruit and vegetable markets: controlling the source of goods.
  • Chain supermarkets and fresh food e-commerce platforms: Quality verification before products are placed on the shelves.
  • Catering Company / School Lunch: Ensuring Food Safety to Provide Peace of Mind for Parents and Consumers.
  • Food processing plants: Rapid initial screening for raw material acceptance.

5.Q&A

Q1:How accurate is this reagent kit? How does it differ from laboratory testing?

A This reagent employs the internationally recognized "biochemical method" known as the "enzyme inhibition rate method", which is characterized by high sensitivity, rapid results, and low cost.

  • Biochemical Method (This Product): This rapid screening technique can qualitatively detect high-risk pesticide residues in a short time, making it ideal for large-scale preliminary screening.
  • Chemical Method (Laboratory): This approach employs advanced instruments, such as LC-MS/MS, for quantitative analysis, enabling precise identification of pesticide types and concentrations. However, it is time-consuming and costly. 
    Recommended Strategy: Use this method for routine, high-frequency screening. If abnormalities are detected, send the samples to the laboratory for confirmatory testing. This approach represents the most cost-effective model for food safety management.

Q2:Can all pesticides be detected?

AThis kit is designed for the highest-risk categories, primarily targeting the two most toxic neurotoxic pesticides to humans: **organophosphates and carbamates**. These pesticides inhibit enzymes involved in neurotransmission, enabling rapid screening with this reagent.

Fungicides, herbicides, and other insecticides with different mechanisms of action are not included in this detection range because they do not inhibit enzyme activity.

Helpful Reminder: If you require testing for fungicides, herbicides, or other specific pesticide categories, our company offers a variety of professional testing products. Please refer to the "Other Product Series" on our website or contact us directly. We will recommend the most suitable testing solution for you.

Q3:What should I do if the test result is "positive" (unacceptable)?

A If the test results indicate that enzyme activity is inhibited (no color change or only a slight color change), the following steps are recommended:

  1. Repeat testing: Use the same sample to retest in order to eliminate operational errors.
  2. Enhanced Cleaning and Retesting: If the produce is intended for personal consumption, it is recommended to extend the washing time under running water or to peel the fruit. Please note that after cleaning, a second test is essential. If the retest result remains positive (unqualified), it is strongly advised, for health and safety reasons, not to consume this batch of fruits and vegetables.
  3. Returning or Re-inspecting at the Source: If the batch of ingredients was purchased, its use should be suspended immediately. The batch should be sent to a third-party professional laboratory for precise analysis to serve as a basis for return or compensation.

Q4:Do reagents need to be frozen? What is their shelf life?

A Yes, because the core component of the reagent is an enzyme, it is quite sensitive to temperature.

  • Storage Method: It is recommended to store the product at 0°C in a frozen environment (or follow the package instructions). Avoid exposure to high temperatures and direct sunlight to preserve the optimal activity of the enzymes.
  • Before use: After removing it from the refrigerator, allow it to reach room temperature before operating.

Q5:Does handling this reagent require professional expertise?

A You do not need a degree in chemistry or laboratory testing; however, to ensure the accuracy and reliability of your test data, we recommend completing approximately one day of basic training. During this brief training, you will learn how to use micropipettes, apply standard extraction techniques, and understand the criteria for interpreting results. After completing this standardized course, even individuals without an undergraduate degree can attain the same level of operational proficiency as professional laboratory technicians and independently conduct accurate screenings.